Cervical shortening is a recognised risk factor for pre-term birth. The vaginal microbiome plays an essential role in pregnancy and in maternal and foetal outcomes. We studied the vaginal microbiome in 68 women with singleton gestation and a cervical length <25 mm and in 29 pregnant women with a cervix >25 mm in the second or early third trimester. Illumina protocol 16S Metagenomic Sequencing Library Preparation was used to detail amplified 16SrRNA gene. Statistical analyses were performed in R environment. Firmicutes was the phylum most represented in all pregnant women. The mean relative abundance of Proteobacteria and Actinobacteriota was higher in women with a short cervix. Bacterial abundance was higher in women with a normal length cervix compared to the group of women with a short cervix. Nonetheless, a significant enrichment in bacterial taxa poorly represented in vaginal microbiome was observed in the group of women with a short cervix. Staphylococcus and Pseudomonas, taxa usually found in aerobic vaginitis, were more common in women with a short cervix compared with the control group, while Lactobacillus iners and Bifidobacterium were associated with a normal cervical.

Vaginal Microbiome in Pregnant Women with and without Short Cervix / Angela Silvano , Niccolò Meriggi, Sonia Renzi, Viola Seravalli , Maria Gabriella Torcia , Duccio Cavalieri, Mariarosaria Di Tommaso. - In: NUTRIENTS. - ISSN 2072-6643. - ELETTRONICO. - (2023), pp. 0-0.

Vaginal Microbiome in Pregnant Women with and without Short Cervix

Angela Silvano;Sonia Renzi;Viola Seravalli;Maria Gabriella Torcia;Mariarosaria Di Tommaso
2023

Abstract

Cervical shortening is a recognised risk factor for pre-term birth. The vaginal microbiome plays an essential role in pregnancy and in maternal and foetal outcomes. We studied the vaginal microbiome in 68 women with singleton gestation and a cervical length <25 mm and in 29 pregnant women with a cervix >25 mm in the second or early third trimester. Illumina protocol 16S Metagenomic Sequencing Library Preparation was used to detail amplified 16SrRNA gene. Statistical analyses were performed in R environment. Firmicutes was the phylum most represented in all pregnant women. The mean relative abundance of Proteobacteria and Actinobacteriota was higher in women with a short cervix. Bacterial abundance was higher in women with a normal length cervix compared to the group of women with a short cervix. Nonetheless, a significant enrichment in bacterial taxa poorly represented in vaginal microbiome was observed in the group of women with a short cervix. Staphylococcus and Pseudomonas, taxa usually found in aerobic vaginitis, were more common in women with a short cervix compared with the control group, while Lactobacillus iners and Bifidobacterium were associated with a normal cervical.
2023
0
0
Angela Silvano , Niccolò Meriggi, Sonia Renzi, Viola Seravalli , Maria Gabriella Torcia , Duccio Cavalieri, Mariarosaria Di Tommaso
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1310281
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