A faster healing process was observed in superficial skin wounds after irradiation with the EMOLED photocoagulator. The instrument consists of a compact handheld photocoagulation device, useful for inducing coagulation in superficial abrasions. In this work we present the results of an in vivo study, in a murine model. Two superficial wounds were produced on the back of 12 mice: one area was left untreated, the other one was treated with EMOLED. Healthy skin was used as a control. The animals were sacrificed 3 hours, 12 hours, 1 day, 6 day after treatment. The treatment effects on back skin was monitored by visual observations, histopathological analysis, immuno-histochemical analysis, and nonlinear microscopic imaging performed at each follow up time, finding no adverse reactions and no thermal damage in both treated areas and surrounding tissues. In addition, a faster healing process, a reduced inflammatory response, a higher collagen content, and a better-recovered skin morphology was evidenced in the treated tissue with respect to the untreated tissue. These morphological features were characterized by means of immuno-histochemical analysis, aimed at imaging fibroblasts and myofibroblasts, and by SHG microscopy, aimed at characterizing collagen organization, demonstrating a fully recovered aspect of dermis as well as a faster neocollagenesis in the treated regions. This study demonstrates that the selective photothermal effect we used for inducing immediate coagulation in superficial wounds is associated to a minimal inflammatory response, which provides reduced recovery times and improved healing process. © 2015 SPIE-OSA.

Healing process study in murine skin superficial wounds treated with the blue LED photocoagulator "EMOLED" / Rossi F, Cicchi R, Tatini F, Bacci S, Alfieri D, De Siena G, Pavone FS, Pini R. - In: PROGRESS IN BIOMEDICAL OPTICS AND IMAGING. - ISSN 1605-7422. - STAMPA. - (2014), pp. 95240-95245. (Intervento presentato al convegno Opt. InfoBase Conf. Pap) [10.1117/12.2183670].

Healing process study in murine skin superficial wounds treated with the blue LED photocoagulator "EMOLED"

Rossi F
Membro del Collaboration Group
;
Cicchi R
Membro del Collaboration Group
;
Bacci S
Membro del Collaboration Group
;
Alfieri D
Membro del Collaboration Group
;
De Siena G
Membro del Collaboration Group
;
Pavone FS
Membro del Collaboration Group
;
Pini R
Membro del Collaboration Group
2014

Abstract

A faster healing process was observed in superficial skin wounds after irradiation with the EMOLED photocoagulator. The instrument consists of a compact handheld photocoagulation device, useful for inducing coagulation in superficial abrasions. In this work we present the results of an in vivo study, in a murine model. Two superficial wounds were produced on the back of 12 mice: one area was left untreated, the other one was treated with EMOLED. Healthy skin was used as a control. The animals were sacrificed 3 hours, 12 hours, 1 day, 6 day after treatment. The treatment effects on back skin was monitored by visual observations, histopathological analysis, immuno-histochemical analysis, and nonlinear microscopic imaging performed at each follow up time, finding no adverse reactions and no thermal damage in both treated areas and surrounding tissues. In addition, a faster healing process, a reduced inflammatory response, a higher collagen content, and a better-recovered skin morphology was evidenced in the treated tissue with respect to the untreated tissue. These morphological features were characterized by means of immuno-histochemical analysis, aimed at imaging fibroblasts and myofibroblasts, and by SHG microscopy, aimed at characterizing collagen organization, demonstrating a fully recovered aspect of dermis as well as a faster neocollagenesis in the treated regions. This study demonstrates that the selective photothermal effect we used for inducing immediate coagulation in superficial wounds is associated to a minimal inflammatory response, which provides reduced recovery times and improved healing process. © 2015 SPIE-OSA.
2014
Optics InfoBase Conference Papers
Opt. InfoBase Conf. Pap
Rossi F, Cicchi R, Tatini F, Bacci S, Alfieri D, De Siena G, Pavone FS, Pini R
File in questo prodotto:
File Dimensione Formato  
62A) SPIE BIOS 9542 14.pdf

accesso aperto

Tipologia: Pdf editoriale (Version of record)
Licenza: DRM non definito
Dimensione 541.69 kB
Formato Adobe PDF
541.69 kB Adobe PDF

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1311401
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 6
social impact