Skin is the largest organ of the body, responsible for thermoregulation and barrier against external agents, including bacteria. Wounds cause loss of skin integrity and can also lead to severe pathologies. For these reasons, efficient treatment of skin wounds is necessary, especially when they become chronic or appear in subjects with comorbidity. Blue LED light photobiomodulation is successfully used in wound management. Although the mechanism of action is still unclear, many studies have been conducted, and some target molecules of blue light are unanimously recognised. Among them, Cytochrome C oxidase is included. For this reason, mitochondria represent a target organelle for blue light radiation. Mitochondria are involved in redox signaling and in maintaining the balance of reactive oxygen species (ROS), essential for several vital functions such as calcium homeostasis. Therefore, studying the effects of blue light on mitochondria may be helpful in identifying a therapeutic dose exploitable in clinical practice. Whit this purpose, primary cultures of human dermal fibroblasts were obtained, and a blue LED light device (410-430 nm in emission, 1 W optical emission power) was used. Three doses of blue light (4, 21, 42 J/cm2) were applied once. Electron microscopy was used to reveal mitochondrial morphology before and after irradiation, while confocal microscopy was used to reveal ROS concentration. Our results demonstrated that blue light stimulates ROS dose-dependently, and mitochondria are subject to morphological changes. Future studies will be devoted to determining whether the change in morphology is also related to changes in function.

Blue LED light affects mitochondria and modulates reactive oxygen species: preliminary in vitro results / Giada Magni , Francesca Tatini , Stefano Bacci , Francesca Rossi. - In: PROGRESS IN BIOMEDICAL OPTICS AND IMAGING. - ISSN 1605-7422. - STAMPA. - 12627:(2023), pp. 1262714-1262717. (Intervento presentato al convegno Translational Biophotonics: Diagnostics and Therapeutics III, 1262714 (11 August 2023)) [10.1117/12.2671071].

Blue LED light affects mitochondria and modulates reactive oxygen species: preliminary in vitro results

Giada Magni
Membro del Collaboration Group
;
Stefano Bacci
Membro del Collaboration Group
;
2023

Abstract

Skin is the largest organ of the body, responsible for thermoregulation and barrier against external agents, including bacteria. Wounds cause loss of skin integrity and can also lead to severe pathologies. For these reasons, efficient treatment of skin wounds is necessary, especially when they become chronic or appear in subjects with comorbidity. Blue LED light photobiomodulation is successfully used in wound management. Although the mechanism of action is still unclear, many studies have been conducted, and some target molecules of blue light are unanimously recognised. Among them, Cytochrome C oxidase is included. For this reason, mitochondria represent a target organelle for blue light radiation. Mitochondria are involved in redox signaling and in maintaining the balance of reactive oxygen species (ROS), essential for several vital functions such as calcium homeostasis. Therefore, studying the effects of blue light on mitochondria may be helpful in identifying a therapeutic dose exploitable in clinical practice. Whit this purpose, primary cultures of human dermal fibroblasts were obtained, and a blue LED light device (410-430 nm in emission, 1 W optical emission power) was used. Three doses of blue light (4, 21, 42 J/cm2) were applied once. Electron microscopy was used to reveal mitochondrial morphology before and after irradiation, while confocal microscopy was used to reveal ROS concentration. Our results demonstrated that blue light stimulates ROS dose-dependently, and mitochondria are subject to morphological changes. Future studies will be devoted to determining whether the change in morphology is also related to changes in function.
2023
European Conferences on Biomedical Optics, 2023, Munich, Germany
Translational Biophotonics: Diagnostics and Therapeutics III, 1262714 (11 August 2023)
Goal 4: Quality education
Giada Magni , Francesca Tatini , Stefano Bacci , Francesca Rossi
File in questo prodotto:
File Dimensione Formato  
103) SPIE BIOS 12627 23.pdf

Accesso chiuso

Tipologia: Pdf editoriale (Version of record)
Licenza: Tutti i diritti riservati
Dimensione 595 kB
Formato Adobe PDF
595 kB Adobe PDF   Richiedi una copia

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1324635
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 0
social impact