3D reconstruction of the human brain at high resolution is one of the most important challenges of neuroscience. We present a new clearing method named SHORT that in combination with an advanced double-view light-sheet fluorescence microscope and an automated machine-learning based images analysis allow to perform volumetric study of the human brain. We applied our methodology to a Broca's area block of 4 x 4 x 2 cm3, demonstrating the possibility of obtaining a fast 3D reconstruction of the human brain at high-resolution, paving the way to the possibility of finally mapping a comprehensive atlas of the human brain

High-resolution human brain 3D reconstruction with light-sheet fluorescence microscopy / Costantini, I; Scardigli, M; Ramazzotti, J; Brady, N; Cheli, F; Mazzamuto, G; Castelli, F; Checcucci, C; Silvestri, L; Frasconi, P; Pavone, FS. - In: PROGRESS IN BIOMEDICAL OPTICS AND IMAGING. - ISSN 1605-7422. - ELETTRONICO. - 12365:(2023), pp. 0-0. (Intervento presentato al convegno SPIE BIOS 2023 tenutosi a San Francisco, USA) [10.1117/12.2649323].

High-resolution human brain 3D reconstruction with light-sheet fluorescence microscopy

Costantini, I
;
Scardigli, M;Ramazzotti, J;Brady, N;Cheli, F;Mazzamuto, G;Castelli, F;Checcucci, C;Silvestri, L;Frasconi, P;Pavone, FS
2023

Abstract

3D reconstruction of the human brain at high resolution is one of the most important challenges of neuroscience. We present a new clearing method named SHORT that in combination with an advanced double-view light-sheet fluorescence microscope and an automated machine-learning based images analysis allow to perform volumetric study of the human brain. We applied our methodology to a Broca's area block of 4 x 4 x 2 cm3, demonstrating the possibility of obtaining a fast 3D reconstruction of the human brain at high-resolution, paving the way to the possibility of finally mapping a comprehensive atlas of the human brain
2023
Neural Imaging and Sensing 2023
SPIE BIOS 2023
San Francisco, USA
Costantini, I; Scardigli, M; Ramazzotti, J; Brady, N; Cheli, F; Mazzamuto, G; Castelli, F; Checcucci, C; Silvestri, L; Frasconi, P; Pavone, FS...espandi
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1330432
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