We report the development of a novel, to the best of our knowledge, fiber-based system to realize coregistered simultaneous acquisition of fluorescence lifetime (FL) data and Raman spectra from the same area. FL measurements by means of time-correlated single photon counting are realized with periodic out-of-phase external illumination of the field of view, enabling acquisition of data under bright illumination of the specimen. Raman measurements in the near-infrared are realized asynchronously. We present a detailed characterization of this technique and validate its potential to report intrinsic contrast. Fiber-based FL and Raman maps report complementary structural, compositional, and molecular contrast in biological tissues with diverse compositional features. (C) 2020 Optical Society of America
Simultaneous fluorescence lifetime and Raman fiber-based mapping of tissues / Lagarto, João L; Shcheslavskiy, Vladislav; Saverio Pavone, Francesco; Cicchi, Riccardo. - In: OPTICS LETTERS. - ISSN 0146-9592. - ELETTRONICO. - 45:(2020), pp. 2247-2250. [10.1364/OL.389300]
Simultaneous fluorescence lifetime and Raman fiber-based mapping of tissues
Saverio Pavone, Francesco;Cicchi, Riccardo
2020
Abstract
We report the development of a novel, to the best of our knowledge, fiber-based system to realize coregistered simultaneous acquisition of fluorescence lifetime (FL) data and Raman spectra from the same area. FL measurements by means of time-correlated single photon counting are realized with periodic out-of-phase external illumination of the field of view, enabling acquisition of data under bright illumination of the specimen. Raman measurements in the near-infrared are realized asynchronously. We present a detailed characterization of this technique and validate its potential to report intrinsic contrast. Fiber-based FL and Raman maps report complementary structural, compositional, and molecular contrast in biological tissues with diverse compositional features. (C) 2020 Optical Society of AmericaI documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.