The aim of this work is to reconstruct the 3D organization of neurons in a left hippocampus from sub-cellular resolution images obtained by a custom-made dual view inverted light sheet fluorescence microscope. The left hippocampus of a 99-year old woman, not affected by Alzheimer’s disease, nor hypertension, but presenting a cognitive decline, obtained from the University of Tours was analyzed. The sample was cut into 72 slices of 500 um thickness before imaging. Each slice was cleared with the SWITCH/TDE method and stained with NeuN (all neurons) and GAD67 (inhibitory neurons). Imaging was performed at a resolution of 1.1 x 1.1 x 3.8 μm³.

Layer-specific excitatory and inhibitory neuronal maps of hippocampus (v1.1) / I. Costantini; G. Mazzamuto; L. Pesce; V. Gavryusev; A. Laurino; M. Scardigli; F. Pavone. - ELETTRONICO. - (2021). [10.25493/md0e-bw5]

Layer-specific excitatory and inhibitory neuronal maps of hippocampus (v1.1)

I. Costantini
;
G. Mazzamuto;L. Pesce;V. Gavryusev;A. Laurino;M. Scardigli;F. Pavone
2021

Abstract

The aim of this work is to reconstruct the 3D organization of neurons in a left hippocampus from sub-cellular resolution images obtained by a custom-made dual view inverted light sheet fluorescence microscope. The left hippocampus of a 99-year old woman, not affected by Alzheimer’s disease, nor hypertension, but presenting a cognitive decline, obtained from the University of Tours was analyzed. The sample was cut into 72 slices of 500 um thickness before imaging. Each slice was cleared with the SWITCH/TDE method and stained with NeuN (all neurons) and GAD67 (inhibitory neurons). Imaging was performed at a resolution of 1.1 x 1.1 x 3.8 μm³.
2021
I. Costantini; G. Mazzamuto; L. Pesce; V. Gavryusev; A. Laurino; M. Scardigli; F. Pavone
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1348491
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