Magnetic resonance imaging (MRI) is used to establish a macroscopic reference coordinate system of laminar and cytoarchitectural boundaries. Cell counting is obtained with both traditional immunohistochemistry, to provide a stereological gold standard, and with a custom-made inverted confocal light-sheet fluorescence microscope (LSM) for 3D imaging at cellular resolution. Finally, polarization-sensitive optical coherence tomography (PSOCT) enables registration of the distorted histological cell typing obtained with LSM to the MRI-based atlas coordinate system.
Human brain cell census for BA 44/45 / Mazzamuto, G., Costantini, I., Gavryusev, V., Castelli, F.M., Pesce, L., Scardigli, M., Pavone, F.S., Roffilli, M., Silvestri, L., Brady, N., Ramazzotti, J., Hof, P.R., Boas, D.A., Fischl, B., Morgan, L., Yang, J., Chang, S., Laffey, J., Magnain, C., Varadarajan, D., et al.. - ELETTRONICO. - (2023).
Human brain cell census for BA 44/45
Mazzamuto, Giacomo;Costantini, Irene
;Gavryusev, Vladislav;Castelli, Filippo Maria;Pesce, Luca;Scardigli, Marina;Pavone, Francesco Saverio;Silvestri, Ludovico;Brady, Niamh;Ramazzotti, Josephine;
2023
Abstract
Magnetic resonance imaging (MRI) is used to establish a macroscopic reference coordinate system of laminar and cytoarchitectural boundaries. Cell counting is obtained with both traditional immunohistochemistry, to provide a stereological gold standard, and with a custom-made inverted confocal light-sheet fluorescence microscope (LSM) for 3D imaging at cellular resolution. Finally, polarization-sensitive optical coherence tomography (PSOCT) enables registration of the distorted histological cell typing obtained with LSM to the MRI-based atlas coordinate system.
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