Magnetic resonance imaging (MRI) is used to establish a macroscopic reference coordinate system of laminar and cytoarchitectural boundaries. Cell counting is obtained with both traditional immunohistochemistry, to provide a stereological gold standard, and with a custom-made inverted confocal light-sheet fluorescence microscope (LSM) for 3D imaging at cellular resolution. Finally, polarization-sensitive optical coherence tomography (PSOCT) enables registration of the distorted histological cell typing obtained with LSM to the MRI-based atlas coordinate system.
Human brain cell census for BA 44/45 / Mazzamuto, Giacomo; Costantini, Irene; Gavryusev, Vladislav; Castelli, Filippo Maria; Pesce, Luca; Scardigli, Marina; Pavone, Francesco Saverio; Roffilli, Matteo; Silvestri, Ludovico; Brady, Niamh; Ramazzotti, Josephine; Hof, Patrick R.; Boas, David A.; Fischl, Bruce; Morgan, Leah; Yang, Jiarui; Chang, Shuaibin; Laffey, Jessie; Magnain, Caroline; Varadarajan, Divya; Wang, Hui; Frost, Robert; Kouwe, Andre van der; Player, Allison Stevens; Atzeni, Alessia; Gonzalez, Juan Eugenio Iglesias; Balbastre, Yael; Vera, Matthew; Cordero, Devani; Nestor, Kimberly; Ammon, William; Nolan, Jackson; Mora, Jocelyn; Pallares, Erendira Garcia; Augustinack, Jean; Diamond, Bram; Fogarty, Morgan; Boyd, Emma; Varghese, Merina; Dalca, Adrian V.; Edlow, Brian; Frosche, Matthew; Wicinski, Bridget; Chen, I-Chun Anderson. - ELETTRONICO. - (2023).
Human brain cell census for BA 44/45
Mazzamuto, Giacomo;Costantini, Irene
;Gavryusev, Vladislav;Castelli, Filippo Maria;Pesce, Luca;Scardigli, Marina;Pavone, Francesco Saverio;Silvestri, Ludovico;Brady, Niamh;Ramazzotti, Josephine;
2023
Abstract
Magnetic resonance imaging (MRI) is used to establish a macroscopic reference coordinate system of laminar and cytoarchitectural boundaries. Cell counting is obtained with both traditional immunohistochemistry, to provide a stereological gold standard, and with a custom-made inverted confocal light-sheet fluorescence microscope (LSM) for 3D imaging at cellular resolution. Finally, polarization-sensitive optical coherence tomography (PSOCT) enables registration of the distorted histological cell typing obtained with LSM to the MRI-based atlas coordinate system.
I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
