Epigenetic changes induced by chronic and acute chromium stress treatments in Arabidopsis thaliana identified by the MSAP-Seq

In this study, the transgenerational epigenetic changes induced by chronic and acute Cr stress treatments in Arabidopsis plants were exploited using the MSAP-Seq approach. First generation plants (F0 plants) were submitted to chronic and acute Cr treatments. Then, F1 plants originating from F0 plants were re-submitted to the same chronic and acute Cr treatments, and genome DNA was purified and analyzed by MSAP-Seq to determine the epigenetic changes caused by Cr stress after a successive treatment over two plant generations. Therefore, the epigenetic modifications imposed by Cr stress treatment in Arabidopsis plants were successfully identified and their effect on plant resilience was addressed and discussed.