Delineation of gastrointestinal tumors biopsies using a fluorescence lifetime imaging optical fiber probe

Autofluorescence spectroscopy has emerged in recent years as a powerful tool to report label-free contrast between normal and diseased tissues, both in vivo and ex-vivo. We report the application of an instrument employing an optical fiber probe and capable of performing real-time autofluorescence lifetime imaging at a macroscopic scale, under bright background conditions. We validate and demonstrate the practicality of this technology to discriminate healthy against neoplastic tissue in freshly excised tumor biopsies. The capability of delineating tumor margins through processing the fluorescence decays in the phasors domain was demonstrated on four different types of cancer, highlighting the broad range of potential clinical applications for the proposed approach. The presented results suggest that our autofluorescence lifetime imaging probe, together with phasor analysis, can offer a real-time tool to observe lifetime contrast on tissues and, thus, is a suitable candidate for improving in situ tissue diagnostics during surgery.The efficient discrimination of a hepatic cancer lesion and its surgical margin using autofluorescence lifetime imaging is presented. White light image of the specimen on the left and the augmented image with fluorescence lifetime data on the right. Our optical fiber-based setup opens new possibilities for using this technology in surgeries across different cancer types. image