FKBP12 is a peptidyl—-prolyl cis–trans isomerase that was recently proposed as a candidate biomarker for cancer, for neurodegenerations and for anti–rejection therapy after organ transplant. We designed and fabricated a nanosensor platform for the rapid and efficient determination of FKBP12 concentration in biological fluids exploiting anisotropic silver nanoparticles (AgNps) to enhance the capabilities of Quartz Crystal Microbalance (QCM) detection. To this end, the QCM sensor was coated with a compact array of AgNPs that were further functionalized with a Self–Assembled– Monolayer containing a synthetic receptor, GPS–SH1, designed and synthesized specifically to selectively bind FKBP12. Silver nanoflowers, AgNFs, and silver dendrites, AgNDs, were prepared by electrodeposition and characterized by means of UV–Vis spectroscopy, Scanning Electron Microscopy (SEM), QCM and water contact angle (CA). The AgNPs@Au/GPS–SH1–functionalized QCM sensors were used to detect increasing concentrations of FKBP12 in solution; the results showed that the use of AgNDs significantly enhanced the sensitivity of the sensor with respect to flat Ag sensor chips, allowing the detection of FKBP12 at sub–picomolar concentrations.

Silver Nanostructures for Determination of FKBP12 Protein † / Bartolini C.; Tozzetti M.; Menichetti S.; Caminati G.. - In: ENGINEERING PROCEEDINGS. - ISSN 2673-4591. - STAMPA. - 73:(2024), pp. 9.1-9.10. [10.3390/engproc2024073009]

Silver Nanostructures for Determination of FKBP12 Protein †

Bartolini C.;Tozzetti M.;Menichetti S.;Caminati G.
2024

Abstract

FKBP12 is a peptidyl—-prolyl cis–trans isomerase that was recently proposed as a candidate biomarker for cancer, for neurodegenerations and for anti–rejection therapy after organ transplant. We designed and fabricated a nanosensor platform for the rapid and efficient determination of FKBP12 concentration in biological fluids exploiting anisotropic silver nanoparticles (AgNps) to enhance the capabilities of Quartz Crystal Microbalance (QCM) detection. To this end, the QCM sensor was coated with a compact array of AgNPs that were further functionalized with a Self–Assembled– Monolayer containing a synthetic receptor, GPS–SH1, designed and synthesized specifically to selectively bind FKBP12. Silver nanoflowers, AgNFs, and silver dendrites, AgNDs, were prepared by electrodeposition and characterized by means of UV–Vis spectroscopy, Scanning Electron Microscopy (SEM), QCM and water contact angle (CA). The AgNPs@Au/GPS–SH1–functionalized QCM sensors were used to detect increasing concentrations of FKBP12 in solution; the results showed that the use of AgNDs significantly enhanced the sensitivity of the sensor with respect to flat Ag sensor chips, allowing the detection of FKBP12 at sub–picomolar concentrations.
2024
73
1
10
Goal 3: Good health and well-being
Bartolini C.; Tozzetti M.; Menichetti S.; Caminati G.
File in questo prodotto:
File Dimensione Formato  
engproc-73-00009.pdf

accesso aperto

Tipologia: Pdf editoriale (Version of record)
Licenza: Creative commons
Dimensione 2 MB
Formato Adobe PDF
2 MB Adobe PDF

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1412374
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? ND
social impact