A device based on the presence of polyphenol oxidase in potato (Solanum tuberosum) tissue was set up to determine catechol. The sensor is realized by coupling a thin slice of potato tissue with a Clark electrode capable of measuring oxygen evolution from a catechol solution. In the concentration range 2.5 X 10(-5) -2.3 X 10(-4) M there is perfect linearity between the electrode signal and catechol concentration in solution. The high stability and the reliability and reproducibility of the results, tested over a period longer than 3 months, and the sensitivity of the device towards mono- and polyphenol derivatives, allow the application of such a system in physiological and pharmacological quality control.
PLANT-TISSUE ELECTRODE FOR THE DETERMINATION OF CATECHOL / BOTRE', Francesco; MAZZEI, Franco; Marco Lanzi; Giampiero Lorenti; Claudio Botre. - In: ANALYTICA CHIMICA ACTA. - ISSN 0003-2670. - STAMPA. - 255:(1991), pp. 59-62. [10.1016/0003-2670(91)85087-9]
PLANT-TISSUE ELECTRODE FOR THE DETERMINATION OF CATECHOL
BOTRE', Francesco;
1991
Abstract
A device based on the presence of polyphenol oxidase in potato (Solanum tuberosum) tissue was set up to determine catechol. The sensor is realized by coupling a thin slice of potato tissue with a Clark electrode capable of measuring oxygen evolution from a catechol solution. In the concentration range 2.5 X 10(-5) -2.3 X 10(-4) M there is perfect linearity between the electrode signal and catechol concentration in solution. The high stability and the reliability and reproducibility of the results, tested over a period longer than 3 months, and the sensitivity of the device towards mono- and polyphenol derivatives, allow the application of such a system in physiological and pharmacological quality control.
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