Fast screening for liposome encapsulated hemoglobin (LEH) using ADVIA 120 flow cytometry-based hematological analyzer

The artificial enhancing of the uptake, transport or delivery of oxygen, including the intake of modified hemoglobin products (e.g. HBOCs) is a prohibited method included in the WADA List of Prohibited Substances (M1. Manipulation of Blood and Blood Components). In this work we present a fast screening to detect liposome encapsulated hemoglobins (LEH). LEH can be detected in serum/plasma using flow cytometry-based techniques with a dual color staining procedure that requires several hours for sample preparation and analysis. The aim of this work is to test the potential of a cytometry-based hematological analyzer such as ADVIA 120 (Siemens) for a rapid screening of the presence of liposomes within a serum/plasma sample.