We describe primers and PCR conditions to amplify seven tetranucleotide loci and two dinucleotide loci isolated from the Malagasy poison frog Mantella crocea. In 21 individuals from Ambohitantely Reserve, the number of alleles ranged from 3 to 15, observed heterozygosities from 24 to 95%. No evidence for linkage disequilibrium was found, but four loci deviated significantly from Hardy-Weinberg equilibrium, possibly due to presence of null alleles. © Springer Science+Business Media B.V. 2009.
Nine microsatellite loci for the Malagasy Poison Frogs (Mantella) / Hauswaldt, J. Susanne; Crottini, Angelica; Vences, Miguel. - In: CONSERVATION GENETICS RESOURCES. - ISSN 1877-7252. - ELETTRONICO. - 1:(2009), pp. 269-271. [10.1007/s12686-009-9066-z]
Nine microsatellite loci for the Malagasy Poison Frogs (Mantella)
Crottini, Angelica;
2009
Abstract
We describe primers and PCR conditions to amplify seven tetranucleotide loci and two dinucleotide loci isolated from the Malagasy poison frog Mantella crocea. In 21 individuals from Ambohitantely Reserve, the number of alleles ranged from 3 to 15, observed heterozygosities from 24 to 95%. No evidence for linkage disequilibrium was found, but four loci deviated significantly from Hardy-Weinberg equilibrium, possibly due to presence of null alleles. © Springer Science+Business Media B.V. 2009.
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