Cyto- and myeloarchitectonics characterization of human cortex and brainstem with light sheet fluorescence microscopy

Costantini, I.; Sorelli, M.; Di Meo, D.; Bradley, S.; Ramazzotti, J.; Lorenzon, B.; Cheli, F.; Perego, L.; Mazzamuto, G.; Checcucci, C.; Frasconi, P.; Pavone, F. S.

doi:10.1117/12.3041140

Characterizing the three-dimensional (3D) structure of the human brain is complex. The use of light sheet fluorescence microscopy (LSFM) has enabled significant progress, allowing the study of brain architecture with unprecedented resolution. We combined LSFM, tissue clearing protocols, and advanced bioimage analysis to create detailed maps of neurons and fiber orientation. Using the SHORT method and a double-view LSFM microscope, along with automated tools BCFindv2 and Foa3D, we analyzed Broca's area and the brainstem. This work demonstrates the feasibility of fast, high-resolution 3D reconstructions of the human brain.

Cyto- and myeloarchitectonics characterization of human cortex and brainstem with light sheet fluorescence microscopy / Costantini I.; Sorelli M.; DI Meo D.; Bradley S.; Ramazzotti J.; Lorenzon B.; Cheli F.; Perego L.; Mazzamuto G.; Checcucci C.; Frasconi P.; Pavone F.S.. - In: PROGRESS IN BIOMEDICAL OPTICS AND IMAGING. - ISSN 1605-7422. - ELETTRONICO. - 13303:(2025), pp. 0-0. (Intervento presentato al convegno Neural Imaging and Sensing 2025 tenutosi a usa nel 2025) [10.1117/12.3041140].