Using the SHORT clearing method in combination with an advanced double-view light-sheet fluorescence microscope and an automated machine-learning-based tool we performed 3D reconstruction of a whole human Broca’s area at micrometer resolution.
Light-sheet fluorescence microscopy for 3D reconstruction of human brain / Costantini I.; Di Meo D.; Ramazzotti J.; Cheli F.; Sorelli M.; Mazzamuto G.; Checcucci C.; Frasconi P.; Pavone F.S.. - ELETTRONICO. - (2024), pp. 0-0. (Intervento presentato al convegno Optics and the Brain, BRAIN 2024 - Part of Optica Biophotonics Congress: Biomedical Optics tenutosi a usa nel 2024) [10.1364/brain.2024.bw3c.4].
Light-sheet fluorescence microscopy for 3D reconstruction of human brain
Costantini I.;Di Meo D.;Ramazzotti J.;Cheli F.;Sorelli M.;Mazzamuto G.;Checcucci C.;Frasconi P.;Pavone F. S.
2024
Abstract
Using the SHORT clearing method in combination with an advanced double-view light-sheet fluorescence microscope and an automated machine-learning-based tool we performed 3D reconstruction of a whole human Broca’s area at micrometer resolution.
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