Current advances in aptamer-functionalized DNA nanostructures for pathogenic bacteria detection in food

Pathogenic bacteria are widely present in food and the environment, posing a serious threat to human health. The key to addressing this issue lies in the development of rapid and effective sensors for the sensitive detection of pathogenic bacteria. Aptamer-functionalized DNA nanostructures represent a cutting-edge approach in biosensing, offering high specificity and versatility for pathogenic bacteria detection. By integrating synthetic aptamers with programmable DNA nanostructures, researchers are developing innovative tools that surpass traditional methods in speed, sensitivity, and portability. This review focuses on the design, types, and applications of aptamer-functionalized DNA nanostructures in the field of pathogenic bacteria detection. We provide a detailed analysis of the assembly methods, characteristics, and functionalities of these nanostructures. Additionally, this work summarizes the applications of aptamer-functionalized DNA nano-sensors based on various sensing signals. Finally, we discuss current challenges and future prospects to inspire advancements in this dynamic field.