Side chain isotope labelling is a powerful tool to study protein structure and interactions. 1H,13C labelling of side-chain methyl groups in a deuterated background allows for extremely sensitive NMR experiments, useful to study large molecules that would be otherwise undetectable. On the other hand, side-chain aromatic groups are highly sensitive to the interactions with ligands, drugs, and other proteins. Moreover, such non-uniform labelling schemes greatly simplify the spectra of large proteins.
Towards cost-effective side-chain isotope labelling of proteins expressed in human cells / Rosati Martina, Barbieri Letizia, Hlavac Matcus, Kratzwald Sarah, Lichtenecker Roman, Konrat Robert, Luchinat Enrico, Banci Lucia. - ELETTRONICO. - (2024), pp. 1-3. (Intervento presentato al convegno International Workshop Advanced Isotopic Labelling Methods tenutosi a Grenoble nel 21-31 maggio 2024).
Towards cost-effective side-chain isotope labelling of proteins expressed in human cells
Rosati Martina;Barbieri Letizia;Luchinat Enrico;Banci Lucia
2024
Abstract
Side chain isotope labelling is a powerful tool to study protein structure and interactions. 1H,13C labelling of side-chain methyl groups in a deuterated background allows for extremely sensitive NMR experiments, useful to study large molecules that would be otherwise undetectable. On the other hand, side-chain aromatic groups are highly sensitive to the interactions with ligands, drugs, and other proteins. Moreover, such non-uniform labelling schemes greatly simplify the spectra of large proteins.
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