Insight into Internal Motions and Interactions of Biomolecules in Blood Serum using High-Resolution Relaxometry

This work demonstrates the use of High-Resolution Relaxometry (HRR) to probe molecular dynamics in complex biological mixtures with site-specific precision. By combining a Bruker high-field NMR spectrometer with a Fast-Shuttle-System and a Field-Cycling-Coil, relaxation rates (R₁) were measured across a broad magnetic field range, revealing molecular motions from picoseconds to nanoseconds. Blood serum was studied as a model system, with NMRD profiles obtained for metabolites such as lactate, creatinine, and succinate using a custom Python processing script. The observed changes in rotational correlation times indicate binding interactions, confirming the potential of HRR as a powerful tool for metabolomic and biomedical applications.