We report on the characterization and use of a Multispot Multiphoton Microscope, to investigate calcium dy- namics at intracellular level. We apply this technique to obtain a time resolution of a few milliseconds, even in full frame images at 512×512 pixels, in order to get the most information on the evolution and propagation of ionic calcium waves across adjacent cells in an intact cardiac tissue. Further we report on the progress of development of a Random Access microscope for very high speed all optical electrophysiological signal acquisition in cell networks. Our study opens the way to the investigation of arrhythmogenic disease in animal models at cellular level. © 2013 SPIE.
Detection of calcium waves in mice heart tissue with multispot two-photon imaging / De Mauro C.; Cecchetti C.A.; Alfieri D.; Borile G.; Urbani A.; Mongillo M.; Pavone F.S.. - In: PROGRESS IN BIOMEDICAL OPTICS AND IMAGING. - ISSN 1605-7422. - ELETTRONICO. - 8588:(2013), pp. 0-0. ( Multiphoton Microscopy in the Biomedical Sciences XIII San Francisco, CA, usa 2013) [10.1117/12.2003789].
Detection of calcium waves in mice heart tissue with multispot two-photon imaging
Alfieri D.;Urbani A.;Pavone F. S.
2013
Abstract
We report on the characterization and use of a Multispot Multiphoton Microscope, to investigate calcium dy- namics at intracellular level. We apply this technique to obtain a time resolution of a few milliseconds, even in full frame images at 512×512 pixels, in order to get the most information on the evolution and propagation of ionic calcium waves across adjacent cells in an intact cardiac tissue. Further we report on the progress of development of a Random Access microscope for very high speed all optical electrophysiological signal acquisition in cell networks. Our study opens the way to the investigation of arrhythmogenic disease in animal models at cellular level. © 2013 SPIE.
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