We report on the characterization and use of a Multispot Multiphoton Microscope, to investigate calcium dynamics at intracellular level. Time resolution of a few milliseconds, even in full frame images at 512×512 pixels, is achieved, in order to get the most information on the evolution and propagation of ionic calcium waves across adjacent cells in an intact cardiac tissue. © 2013 OSA-SPIE.
Multispot two-photon imaging of calcium waves dynamics in cardial tissue at 16hz frame rate / De Mauro C.; Cecchetti C.A.; Alfieri D.; Borile G.; Urbani A.; Mongillo M.; Pavone F.S.. - In: PROGRESS IN BIOMEDICAL OPTICS AND IMAGING. - ISSN 1605-7422. - ELETTRONICO. - 8797:(2013), pp. 0-0. ( Advanced Microscopy Techniques III Munich, deu 2013) [10.1117/12.2032499].
Multispot two-photon imaging of calcium waves dynamics in cardial tissue at 16hz frame rate
Alfieri D.;Urbani A.;Pavone F. S.
2013
Abstract
We report on the characterization and use of a Multispot Multiphoton Microscope, to investigate calcium dynamics at intracellular level. Time resolution of a few milliseconds, even in full frame images at 512×512 pixels, is achieved, in order to get the most information on the evolution and propagation of ionic calcium waves across adjacent cells in an intact cardiac tissue. © 2013 OSA-SPIE.
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