We describe an image-based method for single-shot measurement and real-time correction of defocus in an optical microscope. The method is validated both in bright-field, epifluorescence and light-sheet microscopy.
Fast, image-based autofocus system for high-resolution optical microscopy of whole mouse brains / Silvestri L.; Mullenbroich M.C.; Costantini I.; Di Giovanna A.P.; Sacconi L.; Pavone F.S.. - ELETTRONICO. - 2017:(2017), pp. 0-0. ( Bio-Optics: Design and Application, BODA 2017 usa 2017) [10.1364/BODA.2017.JTu4A.8].
Fast, image-based autofocus system for high-resolution optical microscopy of whole mouse brains
Silvestri L.
;Mullenbroich M. C.;Costantini I.;Di Giovanna A. P.;Sacconi L.;Pavone F. S.
2017
Abstract
We describe an image-based method for single-shot measurement and real-time correction of defocus in an optical microscope. The method is validated both in bright-field, epifluorescence and light-sheet microscopy.
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