The lac operon is a well-known example of gene expression regulation, based on the specific interaction of Lac repressor protein (LacI) with its target DNA sequence (operator). We recently developed an ultrafast force-clamp laser trap technique capable of probing molecular interactions with sub-ms temporal resolution, under controlled pN-range forces. With this technique, we tested the interaction of LacI with different DNA constructs. Based on position along the DNA sequence, the observed interactions can be interpreted as specific binding to operator sequences and transient interactions with nonspecific sequences.
Mapping DNA-Lac repressor interaction with ultra-fast optical tweezers / Monico C.; Tempestini A.; Vanzi F.; Pavone F.S.; Capitanio M.. - In: PROGRESS IN BIOMEDICAL OPTICS AND IMAGING. - ISSN 1605-7422. - ELETTRONICO. - 9331:(2015), pp. 0-0. ( Single Molecule Spectroscopy and Superresolution Imaging VIII usa 2015) [10.1117/12.2080347].
Mapping DNA-Lac repressor interaction with ultra-fast optical tweezers
Tempestini A.;Vanzi F.;Pavone F. S.;Capitanio M.
2015
Abstract
The lac operon is a well-known example of gene expression regulation, based on the specific interaction of Lac repressor protein (LacI) with its target DNA sequence (operator). We recently developed an ultrafast force-clamp laser trap technique capable of probing molecular interactions with sub-ms temporal resolution, under controlled pN-range forces. With this technique, we tested the interaction of LacI with different DNA constructs. Based on position along the DNA sequence, the observed interactions can be interpreted as specific binding to operator sequences and transient interactions with nonspecific sequences.
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