Two modifications of the triple-resonance CANCO sequence, designed for backbone assignment in proteins [Angew. Chem. Int. Ed. 43 (2004) 2257], are presented here. These two new sequences display the intra-residue Ca-CO correlation selectively, while in the original sequence both the inter- and the intra-residue correlations were present. In addition, one of the two variants benefits from an improved sensitivity. Both sequences are a useful complement to the CANCO sequence for facile sequence-specific protein assignment by protonless NMR
A selective experiment for the sequential protein backbone assignment from 3D heteronuclear spectra / W.Bermel; I.Bertini; I.C.Felli; R.Pierattelli; P.R.Vasos. - In: JOURNAL OF MAGNETIC RESONANCE. - ISSN 1090-7807. - STAMPA. - 172:(2005), pp. 324-328.
A selective experiment for the sequential protein backbone assignment from 3D heteronuclear spectra
BERTINI, IVANO;FELLI, ISABELLA CATERINA;PIERATTELLI, ROBERTA;
2005
Abstract
Two modifications of the triple-resonance CANCO sequence, designed for backbone assignment in proteins [Angew. Chem. Int. Ed. 43 (2004) 2257], are presented here. These two new sequences display the intra-residue Ca-CO correlation selectively, while in the original sequence both the inter- and the intra-residue correlations were present. In addition, one of the two variants benefits from an improved sensitivity. Both sequences are a useful complement to the CANCO sequence for facile sequence-specific protein assignment by protonless NMR
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