Molecular and biochemical investigation methods have been exploited to evaluate the effects of pig slurry, used for three years as a fertilizer in intensive agriculture, on the presence and activity of chemolithotrophic ammonia oxidizing bacteria, the biocatalysts of the first step in the nitrification process. The evaluation was carried out, on bulk soil, comparing data from the first and the third year of fertilization. Oligonucleotide sequences selected from the 16S rRNA genes of autotrophic ammonia oxidizers have been used as specific PCR amplification primers and probes. The potential nitrification activity (PNA) has been used to determine the effects of swine manure fertilization on the ammonia oxidizing activity. Members of the genera Nitrosomonas and Nitrosospira were always detectable, but the genus Nitrosospira was more represented than Nitrosomonas. The differences between the hybridization signals were correlated with the doses of pig slurry and the number of applications. The mineral (urea) and organic fertilizations showed similar effects on the ammonia oxidisers investigated by molecular and biochemical methods. It was found that additions of swine manure to soil plots increased both potential ammonia-oxidise activity and ammonia oxidiser sequences. Stimulation of the soil ammonia-oxidise activity was due mainly to its effects on the indigenous bacterial population.

Effects of swine manure fertilization on autotrophic ammonia oxidizing bacteria in soil / Ceccherini M.T.; Castaldini M.; Piovanelli C.; Hastings R.C.; McCarthy A.J.; Bazzicalupo M.; Miclaus N.. - In: APPLIED SOIL ECOLOGY. - ISSN 0929-1393. - STAMPA. - 7:(1998), pp. 149-157. [10.1016/S0929-1393(97)00034-6]

Effects of swine manure fertilization on autotrophic ammonia oxidizing bacteria in soil.

CECCHERINI, MARIA TERESA;BAZZICALUPO, MARCO;
1998

Abstract

Molecular and biochemical investigation methods have been exploited to evaluate the effects of pig slurry, used for three years as a fertilizer in intensive agriculture, on the presence and activity of chemolithotrophic ammonia oxidizing bacteria, the biocatalysts of the first step in the nitrification process. The evaluation was carried out, on bulk soil, comparing data from the first and the third year of fertilization. Oligonucleotide sequences selected from the 16S rRNA genes of autotrophic ammonia oxidizers have been used as specific PCR amplification primers and probes. The potential nitrification activity (PNA) has been used to determine the effects of swine manure fertilization on the ammonia oxidizing activity. Members of the genera Nitrosomonas and Nitrosospira were always detectable, but the genus Nitrosospira was more represented than Nitrosomonas. The differences between the hybridization signals were correlated with the doses of pig slurry and the number of applications. The mineral (urea) and organic fertilizations showed similar effects on the ammonia oxidisers investigated by molecular and biochemical methods. It was found that additions of swine manure to soil plots increased both potential ammonia-oxidise activity and ammonia oxidiser sequences. Stimulation of the soil ammonia-oxidise activity was due mainly to its effects on the indigenous bacterial population.
1998
7
149
157
Ceccherini M.T.; Castaldini M.; Piovanelli C.; Hastings R.C.; McCarthy A.J.; Bazzicalupo M.; Miclaus N.
File in questo prodotto:
File Dimensione Formato  
Ceccherini ASE 98.pdf

Accesso chiuso

Tipologia: Pdf editoriale (Version of record)
Licenza: DRM non definito
Dimensione 723 kB
Formato Adobe PDF
723 kB Adobe PDF   Richiedi una copia

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/203265
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 31
  • ???jsp.display-item.citation.isi??? 30
social impact