By using p65 synaptotagmin-1 and fibroblast growth factor (FGF)-1:β- galactosidase (β-gal) NIH 3T3 cell co-transfectants, we demonstrate that a proteolytic fragment consisting of the extravesicular domain of synaptotagmin-1 is released into the extracellular compartment in response to temperature stress with similar kinetics and pharmacological properties as FGF-1:β-gal. Using a deletion mutant that lacks 95 amino acids from the extravesicular domain of synaptotagmin-1, neither synaptotagmin-1 nor FGF- 1:β-gal are able to access the stress-induced release pathway. Furthermore, the p40 extravesicular fragment of synaptotagmin-1 is constitutively released in p40 synaptotagmin-1 NIH 3T3 cell transfectants, and this release is potentiated when the cells are subjected to temperature stress. These data demonstrate that the p40 fragment derived from synaptotagmin-1 is able to utilize the FGF-1 non-classical exocytotic pathway and that the release of FGF-1 is dependent on synaptotagmin-1.
Synaptotagmin-1 is required for fibroblast growth factor-1 release / LaVallee, Theresa M; Tarantini, Francesca; Gamble, Susan; Mouta Carreira, Carla; Jackson, Anthony; Maciag, Thomas. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 1067-8816. - STAMPA. - 273:(1998), pp. 22217-22223.
Synaptotagmin-1 is required for fibroblast growth factor-1 release.
TARANTINI, FRANCESCA;
1998
Abstract
By using p65 synaptotagmin-1 and fibroblast growth factor (FGF)-1:β- galactosidase (β-gal) NIH 3T3 cell co-transfectants, we demonstrate that a proteolytic fragment consisting of the extravesicular domain of synaptotagmin-1 is released into the extracellular compartment in response to temperature stress with similar kinetics and pharmacological properties as FGF-1:β-gal. Using a deletion mutant that lacks 95 amino acids from the extravesicular domain of synaptotagmin-1, neither synaptotagmin-1 nor FGF- 1:β-gal are able to access the stress-induced release pathway. Furthermore, the p40 extravesicular fragment of synaptotagmin-1 is constitutively released in p40 synaptotagmin-1 NIH 3T3 cell transfectants, and this release is potentiated when the cells are subjected to temperature stress. These data demonstrate that the p40 fragment derived from synaptotagmin-1 is able to utilize the FGF-1 non-classical exocytotic pathway and that the release of FGF-1 is dependent on synaptotagmin-1.File | Dimensione | Formato | |
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