In this study, we report that low doses of tumor necrosis factor-alpha (TNFalpha) promote myogenesis in C2C12 myoblasts. Moreover, the cytokine increased sphingosine kinase (SphK) activity and induced SphK1 translocation to membranes. The inhibition of SphK functionality by various approaches abrogated the pro-myogenic effect of TNFalpha. Moreover, silencing of S1P(2) impaired the positive action of TNFalpha on myogenesis. These results represent the first evidence that SphK/S1P(2) axis is required for the regulation of myogenesis by TNFalpha. In view of the physiological role of TNFalpha in muscle regeneration, the present finding reinforces the notion that SphK/S1P(2) signaling is critically implicated in myogenesis.

Tumor necrosis factor-α exerts promyogenic action in C2C12 myoblasts via sphingosine kinase/S1P2 signaling / C. DONATI ; P. NINCHERI ; F. CENCETTI ; E. RAPIZZI ; M. FARNARARO ; P. BRUNI. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 581:(2007), pp. 4384-4388.

Tumor necrosis factor-α exerts promyogenic action in C2C12 myoblasts via sphingosine kinase/S1P2 signaling.

DONATI, CHIARA;CENCETTI, FRANCESCA;RAPIZZI, ELENA;FARNARARO, MARTA;BRUNI, PAOLA
2007

Abstract

In this study, we report that low doses of tumor necrosis factor-alpha (TNFalpha) promote myogenesis in C2C12 myoblasts. Moreover, the cytokine increased sphingosine kinase (SphK) activity and induced SphK1 translocation to membranes. The inhibition of SphK functionality by various approaches abrogated the pro-myogenic effect of TNFalpha. Moreover, silencing of S1P(2) impaired the positive action of TNFalpha on myogenesis. These results represent the first evidence that SphK/S1P(2) axis is required for the regulation of myogenesis by TNFalpha. In view of the physiological role of TNFalpha in muscle regeneration, the present finding reinforces the notion that SphK/S1P(2) signaling is critically implicated in myogenesis.
2007
581
4384
4388
C. DONATI ; P. NINCHERI ; F. CENCETTI ; E. RAPIZZI ; M. FARNARARO ; P. BRUNI
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/250626
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