Selection of background electrolyte for CZE analysis by a chemometric approach: Part II. Separation of a mixture of basic beta-blocker drugs

In the first part of this study a chemometric approach to choose a suitable background electrolyte for CZE analysis was introduced. Two hundred and twenty-two possible electrolytes were previously characterized by means of the descriptors pH, conductivity, ionic strength and relative viscosity and the approach was applied to the separation of a mixture of acidic drugs. In this second part, another application concerning the analysis of basic drugs is presented. The test mixture was made of eight beta-blocker drugs. According to the basic nature of the analytes, the original data set was reduced to a new subset of 117 objects with pH less than or equal to 7, and after computing principal components the new set of objects was represented in a two-dimensional space. Ten objects to be tested in CZE, capable of covering homogeneously the principal component space, were selected by means of Kennard-Stone algorithm. The data set was further reduced around the BGEs which gave the best results, and a new set of electrolytes to be tested was selected. Using pH 4 citrate buffer, an electropherograrn with baseline resolution was obtained in 10 min. A Doehlert design was run to further reduce analysis time, and applying the optimized conditions (voltage, 23 kV; temperature, 26 degrees C) the separation was obtained in about 7 min.