Benzo(a)pyrene diol-epoxide (BPDE)-DNA adducts and aryl hydrocarbon hydroxylase (AHH) activity were determined in white blood cells (WBCs) obtained from non-smokers, ex-smokers and smokers. DNA adduct levels ranged from non-detectable (ND) to 8.8 per 108 nucleosides in all samples analysed. The mean level in WBC from smokers (1.93 +/- 0.55 mean +/- SE) was similar to that from non-smokers (1.18 +/- 0.40) and ex-smokers (1.83 +/- 0.50). The distribution frequency of AHH activity in non-smokers was different from that in smokers, while 50% of non-smokers had AHH activity ranging from ND to 20 fmol mg-1 min-1. This low AHH activity was not found in smokers; no correlation was found between AHH activity and tobacco consumption (r=0.097; P=0.76; n=12). On the contrary, AHH activity was correlated with BPDE-DNA adduct levels in WBCs from the same subjects (r=0.457; P=0.019; n=26). Our findings indicate that there are limitations to the use of WBCs as surrogate cells for determining internal PAH exposure in target organs.

Benzo(a)pyrene diol-epoxide-DNA adduct levels and aryl hydrocarbon hydroxylase (AHH) in human white blood cells from smokers and non-smokers / M. Lodovici; V. Akpan; C. Casalini; L. Nencini; M. Pinzauti; M. Marcoccia; P. Dolara. - In: BIOMARKERS. - ISSN 1354-750X. - STAMPA. - 4:(1999), pp. 272-280.

Benzo(a)pyrene diol-epoxide-DNA adduct levels and aryl hydrocarbon hydroxylase (AHH) in human white blood cells from smokers and non-smokers.

LODOVICI, MAURA;DOLARA, PIERO
1999

Abstract

Benzo(a)pyrene diol-epoxide (BPDE)-DNA adducts and aryl hydrocarbon hydroxylase (AHH) activity were determined in white blood cells (WBCs) obtained from non-smokers, ex-smokers and smokers. DNA adduct levels ranged from non-detectable (ND) to 8.8 per 108 nucleosides in all samples analysed. The mean level in WBC from smokers (1.93 +/- 0.55 mean +/- SE) was similar to that from non-smokers (1.18 +/- 0.40) and ex-smokers (1.83 +/- 0.50). The distribution frequency of AHH activity in non-smokers was different from that in smokers, while 50% of non-smokers had AHH activity ranging from ND to 20 fmol mg-1 min-1. This low AHH activity was not found in smokers; no correlation was found between AHH activity and tobacco consumption (r=0.097; P=0.76; n=12). On the contrary, AHH activity was correlated with BPDE-DNA adduct levels in WBCs from the same subjects (r=0.457; P=0.019; n=26). Our findings indicate that there are limitations to the use of WBCs as surrogate cells for determining internal PAH exposure in target organs.
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272
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M. Lodovici; V. Akpan; C. Casalini; L. Nencini; M. Pinzauti; M. Marcoccia; P. Dolara
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2158/253344
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