Abstract: We report the isolation and characterization of endophytic bacteria, endemic to serpentine outcrops of Central Italy, from a nickel hyperaccumulator plant, Alyssum bertolonii Desv. (Brassicaceae). Eighty-three endophytic bacteria were isolated from roots, stems, and leaves of A. bertolonii and classified by restriction analysis of 16S rDNA (ARDRA) and partial 16S rDNA sequencing in 23 different taxonomic groups. All isolates were then screened for siderophore production and for resistance to heavy metals. One isolate representative of each ARDRA group was then tested for plant tissue colonization ability in sterile culture.Obtained results pointed out that, despite the high concentration of heavy metals present in its tissues, A. bertolonii harbors an endophytic bacterial flora showing a high genetic diversity as well as a high level of resistance to heavy metals that could potentially help plant growth and Ni hyperaccumulation.
Isolation and characterization of endophytic bacteria from the nickel-hyperaccumulator plant Alyssum bertolonii / R. BARZANTI; F. OZINO; M. BAZZICALUPO; R. GABBRIELLI; F. GALARDI; C. GONNELLI; A. MENGONI. - In: MICROBIAL ECOLOGY. - ISSN 0095-3628. - STAMPA. - 53:(2007), pp. 306-316.
Isolation and characterization of endophytic bacteria from the nickel-hyperaccumulator plant Alyssum bertolonii
BAZZICALUPO, MARCO;GABBRIELLI, ROBERTO;GONNELLI, CRISTINA;MENGONI, ALESSIO
2007
Abstract
Abstract: We report the isolation and characterization of endophytic bacteria, endemic to serpentine outcrops of Central Italy, from a nickel hyperaccumulator plant, Alyssum bertolonii Desv. (Brassicaceae). Eighty-three endophytic bacteria were isolated from roots, stems, and leaves of A. bertolonii and classified by restriction analysis of 16S rDNA (ARDRA) and partial 16S rDNA sequencing in 23 different taxonomic groups. All isolates were then screened for siderophore production and for resistance to heavy metals. One isolate representative of each ARDRA group was then tested for plant tissue colonization ability in sterile culture.Obtained results pointed out that, despite the high concentration of heavy metals present in its tissues, A. bertolonii harbors an endophytic bacterial flora showing a high genetic diversity as well as a high level of resistance to heavy metals that could potentially help plant growth and Ni hyperaccumulation.File | Dimensione | Formato | |
---|---|---|---|
Barzanti et al MicrobEcol 2007.pdf
Accesso chiuso
Tipologia:
Versione finale referata (Postprint, Accepted manuscript)
Licenza:
Tutti i diritti riservati
Dimensione
267.87 kB
Formato
Adobe PDF
|
267.87 kB | Adobe PDF | Richiedi una copia |
I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.