Design of an optimal allele-specific oligonucleotide probe for the efficient discrimination of a thermodynamically stable (G*T) mismatch

A general approach that allows reliable detection of a single base-pair mismatch is pre- sented. Specifically, how important a careful design of the allele-specific oligonucleotide (ASO) probe is, was demonstrated for a G·T mispair highly stable from the thermodynamic point of view.