Previous experiments in vitro have demonstrated the ability of acylphosphatase to increase the rate of glucose fermentation in yeast. To evaluate the possibility of increasing fermentation in vivo also, a chemically synthesized DNA sequence coding for human muscle acylphosphatase was expressed at high level in Saccharomyces cerevisiae. Ethanol production was measured in these engineered strains in comparison with a control. Acylphosphatase expression strongly increased the rate of ethanol production both in aerobic and anaerobic culture. This finding may be potentially important for the development of more efficient industrial fermentation processes.
Expression of acylphosphatase in Saccharomyces cerevisiae enhances ethanol fermentation rate / G. RAUGEI;A. MODESTI; F. MAGHERINI; R. MARZOCCHINI; M. VECCHI; G. RAMPONI. - In: BIOCHEMICAL JOURNAL. - ISSN 0264-6021. - ELETTRONICO. - 23:(1996), pp. 273-278.
Expression of acylphosphatase in Saccharomyces cerevisiae enhances ethanol fermentation rate.
RAUGEI, GIOVANNI;MODESTI, ALESSANDRA;MAGHERINI, FRANCESCA;MARZOCCHINI, RICCARDO;RAMPONI, GIAMPIETRO
1996
Abstract
Previous experiments in vitro have demonstrated the ability of acylphosphatase to increase the rate of glucose fermentation in yeast. To evaluate the possibility of increasing fermentation in vivo also, a chemically synthesized DNA sequence coding for human muscle acylphosphatase was expressed at high level in Saccharomyces cerevisiae. Ethanol production was measured in these engineered strains in comparison with a control. Acylphosphatase expression strongly increased the rate of ethanol production both in aerobic and anaerobic culture. This finding may be potentially important for the development of more efficient industrial fermentation processes.I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.