A chemically synthesized polynucleotide sequence coding for bovine liver low molecular weight acid phosphatase (which possesses phosphotyrosine-protein phosphatase activity) has been cloned in a E. coli expression vector. The recombinant protein retains correct affinity for substrate and inhibitors but shows a reduced specific activity.
Chemical synthesis and expression of a gene coding for bovine liver phosphotyrosine-protein phosphatase / G. RAUGEI ; R. MARZOCCHINI; A. MODESTI ; G. RATTI ; G. CAPPUGI ; G. CAMICI ; G. MANAO ; G. RAMPONI. - In: BIOCHEMISTRY INTERNATIONAL. - ISSN 0158-5231. - STAMPA. - 23:(1991), pp. 317-326.
Chemical synthesis and expression of a gene coding for bovine liver phosphotyrosine-protein phosphatase.
RAUGEI, GIOVANNI;MARZOCCHINI, RICCARDO;MODESTI, ALESSANDRA;CAPPUGI, GIANNI;CAMICI, GUIDO;MANAO, GIAMPAOLO;RAMPONI, GIAMPIETRO
1991
Abstract
A chemically synthesized polynucleotide sequence coding for bovine liver low molecular weight acid phosphatase (which possesses phosphotyrosine-protein phosphatase activity) has been cloned in a E. coli expression vector. The recombinant protein retains correct affinity for substrate and inhibitors but shows a reduced specific activity.
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