An electrochemical immunosensor for polychlorinated biphenyl (PCB) detection based on graphite screen-printed low-density arrays and on magnetic beads is reported. The immunological reaction for the detection of PCBs is based on a direct competitive assay using alkaline phosphatase (AP) as enzymatic label. After the immunochemical recognition, the modified magnetic beads are captured by a magnet on the surface of the graphite working electrode. The electrochemical detection is thus achieved through the addition of the AP substrate ( -naphthyl-phosphate). Two different antibodies (sIgG anti-PCB28 and rIgG anti-PCB77) were tested and compared in terms of sensitivity and ability to recognise different congeners. The developed electrochemical magneto-immunosensor (EMI) was successfully combined with solid-phase extraction (SPE) for the analysis of PCBs in milk samples. In spiked samples a recovery of 80% was obtained. The proposed strategy offers great promise for rapid, simple, cost-effective, and on-site analysis of clinical, food and environmental samples, considering also that low-density arrays allow the simultaneous analysis of different processed samples.

Polychlorinated biphenyls (PCBs) detection in milk samples by an electrochemical magneto-immunosensor (EMI) coupled to Solid Phase Extraction (SPE) and disposable low density arrays / S. Centi; E. Silva; S. Laschi; I. Palchetti; M. Mascini. - In: ANALYTICA CHIMICA ACTA. - ISSN 0003-2670. - STAMPA. - 594:(2007), pp. 9-16. [10.1016/j.aca.2007.04.064]

Polychlorinated biphenyls (PCBs) detection in milk samples by an electrochemical magneto-immunosensor (EMI) coupled to Solid Phase Extraction (SPE) and disposable low density arrays

PALCHETTI, ILARIA;
2007

Abstract

An electrochemical immunosensor for polychlorinated biphenyl (PCB) detection based on graphite screen-printed low-density arrays and on magnetic beads is reported. The immunological reaction for the detection of PCBs is based on a direct competitive assay using alkaline phosphatase (AP) as enzymatic label. After the immunochemical recognition, the modified magnetic beads are captured by a magnet on the surface of the graphite working electrode. The electrochemical detection is thus achieved through the addition of the AP substrate ( -naphthyl-phosphate). Two different antibodies (sIgG anti-PCB28 and rIgG anti-PCB77) were tested and compared in terms of sensitivity and ability to recognise different congeners. The developed electrochemical magneto-immunosensor (EMI) was successfully combined with solid-phase extraction (SPE) for the analysis of PCBs in milk samples. In spiked samples a recovery of 80% was obtained. The proposed strategy offers great promise for rapid, simple, cost-effective, and on-site analysis of clinical, food and environmental samples, considering also that low-density arrays allow the simultaneous analysis of different processed samples.
2007
594
9
16
S. Centi; E. Silva; S. Laschi; I. Palchetti; M. Mascini
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/255112
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