Several studies have investigated the adsorption of pure DNA on soil particles and its transformation ability. However, the presence of not purified (dirty) rather than pure DNA is more common in the extracellular soil environment. For this reason, we have investigated the adsorption and binding of dirty DNA on montmorillonite (M) and kaolinite (K) and their transforming ability in comparison to pure DNA. After lysis of Bacillus subtilis cells, induced by KCl, dirty DNA was characterized by the presence of cellular wall debris (cwd) and other constitutional organic components (coc). The dirty DNA was then divided into two fractions, one with cellular wall debris (DNA +cwd) and the other without cellular wall debris (DNA -cwd). B. subtilis BD 1512 (Cm-r) and BD 170 (Cms) were selected as donor and recipient bacteria, respectively, for adsorption and transformation studies. Both cwd and coc seem to facilitate the adsorption of DNA to clay minerals, whereas only cwd promote the DNA binding on clays, protecting also the DNA fragments below 400 bp against nucleases. Both dirty DNA fractions adsorbed and bound on clay minerals were able to transform competent cells.

Adsorption of pure and dirty bacterial DNA on clay minerals and their transformation frequency / G. PIETRAMELLARA; J. ASCHER; M.T. CECCHERINI; P. NANNIPIERI; D. WENDEROTH. - In: BIOLOGY AND FERTILITY OF SOILS. - ISSN 0178-2762. - STAMPA. - 43:(2007), pp. 731-739. [10.1007/s00374-006-0156-8]

Adsorption of pure and dirty bacterial DNA on clay minerals and their transformation frequency

PIETRAMELLARA, GIACOMO;ASCHER, JUDITH;CECCHERINI, MARIA TERESA;NANNIPIERI, PAOLO;
2007

Abstract

Several studies have investigated the adsorption of pure DNA on soil particles and its transformation ability. However, the presence of not purified (dirty) rather than pure DNA is more common in the extracellular soil environment. For this reason, we have investigated the adsorption and binding of dirty DNA on montmorillonite (M) and kaolinite (K) and their transforming ability in comparison to pure DNA. After lysis of Bacillus subtilis cells, induced by KCl, dirty DNA was characterized by the presence of cellular wall debris (cwd) and other constitutional organic components (coc). The dirty DNA was then divided into two fractions, one with cellular wall debris (DNA +cwd) and the other without cellular wall debris (DNA -cwd). B. subtilis BD 1512 (Cm-r) and BD 170 (Cms) were selected as donor and recipient bacteria, respectively, for adsorption and transformation studies. Both cwd and coc seem to facilitate the adsorption of DNA to clay minerals, whereas only cwd promote the DNA binding on clays, protecting also the DNA fragments below 400 bp against nucleases. Both dirty DNA fractions adsorbed and bound on clay minerals were able to transform competent cells.
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731
739
G. PIETRAMELLARA; J. ASCHER; M.T. CECCHERINI; P. NANNIPIERI; D. WENDEROTH
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2158/255481
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