Acylphosphatase was purified from rabbit skeletal muscle by a procedure involving an affinity chromatography step on immunoadsorbent and subsequent ion-exchange chromatography. Three molecular forms with acylphosphatase activity, named Ra1, Ra2, and Ra3, were purified and characterized with respect to molecular weight, amino acid composition, and main kinetic parameters. The amino acid sequence of Ra1 is given in the present paper. The Ra1 form consists of a single polypeptide chain of 98 amino acid residues and contains only one cysteine residue at position 21 that is S-S bound to glutathione. The polypeptide chain has an acetyl group blocking the NH2 terminus. Ra1, Ra2, and Ra3 are compared with the corresponding molecular forms isolated from skeletal muscle of horse and turkey.

Rabbit skeletal muscle acylphosphatase: the amino acid sequence of form Ra1 / G. MANAO ; G. CAMICI ; G. CAPPUGI; M. STEFANI ; G. LIGURI ; A. BERTI ; G. RAMPONI. - In: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS. - ISSN 0003-9861. - STAMPA. - 241(2):(1985), pp. 418-424.

Rabbit skeletal muscle acylphosphatase: the amino acid sequence of form Ra1.

MANAO, GIAMPAOLO;CAMICI, GUIDO;CAPPUGI, GIANNI;STEFANI, MASSIMO;LIGURI, GIANFRANCO;BERTI, ANDREA;RAMPONI, GIAMPIETRO
1985

Abstract

Acylphosphatase was purified from rabbit skeletal muscle by a procedure involving an affinity chromatography step on immunoadsorbent and subsequent ion-exchange chromatography. Three molecular forms with acylphosphatase activity, named Ra1, Ra2, and Ra3, were purified and characterized with respect to molecular weight, amino acid composition, and main kinetic parameters. The amino acid sequence of Ra1 is given in the present paper. The Ra1 form consists of a single polypeptide chain of 98 amino acid residues and contains only one cysteine residue at position 21 that is S-S bound to glutathione. The polypeptide chain has an acetyl group blocking the NH2 terminus. Ra1, Ra2, and Ra3 are compared with the corresponding molecular forms isolated from skeletal muscle of horse and turkey.
1985
241(2)
418
424
G. MANAO ; G. CAMICI ; G. CAPPUGI; M. STEFANI ; G. LIGURI ; A. BERTI ; G. RAMPONI
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/308129
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