An open reading flame encoding a putative acylphosphatase was found in Drosophila melanogaster. The corresponding gene product shows 40% identity and 22 additional amino acid residues at the C-terminus as compared to muscle- and common-type human acylphosphatases. Moreover, all the residues involved in the catalytic mechanism of vertebrate enzymes are conserved in the D. melanogaster acylphosphatase. The D. melanogaster protein and a deletion mutant, similar in length to vertebrate acylphosphatases, were produced by cloning the corresponding cDNA in Escherichia coli. The wild-type enzyme is a protein with a well-established three-dimensional fold and a markedly reduced conformational stability as compared to vertebrate isoenzymes. The specific activity of the enzyme is significantly lower than that found in vertebrate enzymes though the substrate binding capability is basically unaltered. The deletion of 22 residues does not cause a significant change in kcat, while affecting the apparent binding parameters. This work suggests that the genes encoding the vertebrate enzymes originate from an ancestor gene by duplication and subsequent evolution.

DROSOPHILA MELANOGASTER ACYLPHOSPHATASE: A COMMON ANCESTOR FOR ACYLPHOSPHATASE ISOENZYMES OF VERTEBRATE SPECIES / A. PIERI; F. MAGHERINI; G. LIGURI; G. RAUGEI; N. TADDEI; M. BOZZETTI; C. CECCHI; G. RAMPONI;. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 433:(1998), pp. 205-210.

DROSOPHILA MELANOGASTER ACYLPHOSPHATASE: A COMMON ANCESTOR FOR ACYLPHOSPHATASE ISOENZYMES OF VERTEBRATE SPECIES

PIERI, ALESSANDRO;MAGHERINI, FRANCESCA;LIGURI, GIANFRANCO;RAUGEI, GIOVANNI;TADDEI, NICCOLO';CECCHI, CRISTINA;RAMPONI, GIAMPIETRO
1998

Abstract

An open reading flame encoding a putative acylphosphatase was found in Drosophila melanogaster. The corresponding gene product shows 40% identity and 22 additional amino acid residues at the C-terminus as compared to muscle- and common-type human acylphosphatases. Moreover, all the residues involved in the catalytic mechanism of vertebrate enzymes are conserved in the D. melanogaster acylphosphatase. The D. melanogaster protein and a deletion mutant, similar in length to vertebrate acylphosphatases, were produced by cloning the corresponding cDNA in Escherichia coli. The wild-type enzyme is a protein with a well-established three-dimensional fold and a markedly reduced conformational stability as compared to vertebrate isoenzymes. The specific activity of the enzyme is significantly lower than that found in vertebrate enzymes though the substrate binding capability is basically unaltered. The deletion of 22 residues does not cause a significant change in kcat, while affecting the apparent binding parameters. This work suggests that the genes encoding the vertebrate enzymes originate from an ancestor gene by duplication and subsequent evolution.
1998
433
205
210
A. PIERI; F. MAGHERINI; G. LIGURI; G. RAUGEI; N. TADDEI; M. BOZZETTI; C. CECCHI; G. RAMPONI;
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/308699
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