Polar/apolar compounds induce leukemia cell differentiation by modulating cell-surface potential

Arcangeli, Annarosa; Carla', Marcello; Del Bene, M.; Becchetti, A.; Wanke, E.; Olivotto, Massimo

The mechanism of action of polar/apolar inducers of cell differentiation, such as dimethyl sulfoxide and hexamethylene-bisacetamide, is still obscure. In this paper evidence is provided that their effects on murine erythroleukemia cells are modulated by various extracellular cations as a precise function of the cation effects on membrane surface potential. The interfacial effects of the inducers were directly measured on the charged electrode, showing that both dimethyl sulfoxide and hexamethylene-bisacetamide, at the effective concentrations for cell differentiation and within the physiological range of charge density, adsorb at the charged surface and produce a potential shift. A linear correlation was found between this shift and the inducer effects on cell differentiation. Besides offering a different interpretation of the mechanism of action of the inducers, these findings indicate that surface potential has a signaling function. They may also be relevant to cancer treatments based on tumor-cell commitment to terminal differentiation.

trova@unifi

Titolo:	Polar/apolar compounds induce leukemia cell differentiation by modulating cell-surface potential
Autori di Ateneo:	ARCANGELI, ANNAROSA CARLA', MARCELLO M. DEL BENE A. BECCHETTI E. WANKE OLIVOTTO, MASSIMO mostra contributor esterni nascondi contributor esterni
Autori:	ARCANGELI, ANNAROSA; CARLA', MARCELLO; M. DEL BENE; A. BECCHETTI; E. WANKE; OLIVOTTO, MASSIMO
Data di pubblicazione:	1993
Rivista:	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume:	90
Pagina iniziale:	5858
Pagina finale:	5862
Abstract:	The mechanism of action of polar/apolar inducers of cell differentiation, such as dimethyl sulfoxide and hexamethylene-bisacetamide, is still obscure. In this paper evidence is provided that their effects on murine erythroleukemia cells are modulated by various extracellular cations as a precise function of the cation effects on membrane surface potential. The interfacial effects of the inducers were directly measured on the charged electrode, showing that both dimethyl sulfoxide and hexamethylene-bisacetamide, at the effective concentrations for cell differentiation and within the physiological range of charge density, adsorb at the charged surface and produce a potential shift. A linear correlation was found between this shift and the inducer effects on cell differentiation. Besides offering a different interpretation of the mechanism of action of the inducers, these findings indicate that surface potential has a signaling function. They may also be relevant to cancer treatments based on tumor-cell commitment to terminal differentiation.
Handle:	http://hdl.handle.net/2158/308715
Appare nelle tipologie:	1a - Articolo su rivista

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