A database of nonredundant structures of EF-hand domains-i.e., pairs of helix-loop-helix motifs-has been assembled, and the six angles among the four helices re-determined. A principal component analysis of these angles allows us to use two such components (PC1 and PC2) to describe the system retaining 80% of the total variance. A PC2 against PC1 plot representation allows us to represent in a compact way the full range of structural diversity of EF-hand domains, their grouping into protein families, and the variation for each family upon calcium and peptide binding. © 2005 American Chemical Society.
Principal Component Analysis of the Conformational Freedom within the EF-Hand Superfamily / E.Babini; I.Bertini; F.Capozzi; C.Luchinat; A.Quattrone; M.Turano. - In: JOURNAL OF PROTEOME RESEARCH. - ISSN 1535-3893. - STAMPA. - 4:(2005), pp. 1961-1971. [10.1021/pr050148n]
Principal Component Analysis of the Conformational Freedom within the EF-Hand Superfamily
BERTINI, IVANO;LUCHINAT, CLAUDIO;QUATTRONE, ALESSANDRO;
2005
Abstract
A database of nonredundant structures of EF-hand domains-i.e., pairs of helix-loop-helix motifs-has been assembled, and the six angles among the four helices re-determined. A principal component analysis of these angles allows us to use two such components (PC1 and PC2) to describe the system retaining 80% of the total variance. A PC2 against PC1 plot representation allows us to represent in a compact way the full range of structural diversity of EF-hand domains, their grouping into protein families, and the variation for each family upon calcium and peptide binding. © 2005 American Chemical Society.I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.