A comparison of different calibration methods for optical tweezers with the differential interference contrast (DIC) technique was performed to establish the uses and the advantages of each method. A detailed experimental and theoretical analysis of each method was performed with emphasis on the anisotropy involved in the DIC technique and the noise components in the detection. Finally, a time of flight method that permits the reconstruction of the optical potential well was demonstrated.
Calibration of optical tweezers with differential interference contrast signals / M. CAPITANIO, G. ROMANO, R. BALLERINI, D. DUNLAP, L. FINZI, M. GIUNTINI, F.S. PAVONE. - In: REVIEW OF SCIENTIFIC INSTRUMENTS. - ISSN 0034-6748. - STAMPA. - 73:(2002), pp. 1687-1696. [10.1063/1.1460929]
Calibration of optical tweezers with differential interference contrast signals
CAPITANIO, MARCO;ROMANO, GIOVANNI;GIUNTINI, MAURO;PAVONE, FRANCESCO SAVERIO
2002
Abstract
A comparison of different calibration methods for optical tweezers with the differential interference contrast (DIC) technique was performed to establish the uses and the advantages of each method. A detailed experimental and theoretical analysis of each method was performed with emphasis on the anisotropy involved in the DIC technique and the noise components in the detection. Finally, a time of flight method that permits the reconstruction of the optical potential well was demonstrated.
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