A novel system to study the evolution of transcription signals in heterologous systems under selective starvation conditions is described. It is based on the plasmid-mediated transfer of his biosynthetic genes from Azospirillum brasilense into a heterologous Escherichia coli mutant population lacking histidine biosynthetic ability. We show that under highly selective stressful conditions, genetic changes in the donor plasmid lead to mutated sequences that are efficiently recognized as promoters by the E. coli RNA polymerase.
Heterologous gene expression in an Escherichia coli population under starvation stress conditions / R. Fani; R. Gallo; S. Fancelli; E. Mori; E. Tamburini; A. Lazcano. - In: JOURNAL OF MOLECULAR EVOLUTION. - ISSN 0022-2844. - STAMPA. - 47:(1998), pp. 363-368.
Heterologous gene expression in an Escherichia coli population under starvation stress conditions.
FANI, RENATO;
1998
Abstract
A novel system to study the evolution of transcription signals in heterologous systems under selective starvation conditions is described. It is based on the plasmid-mediated transfer of his biosynthetic genes from Azospirillum brasilense into a heterologous Escherichia coli mutant population lacking histidine biosynthetic ability. We show that under highly selective stressful conditions, genetic changes in the donor plasmid lead to mutated sequences that are efficiently recognized as promoters by the E. coli RNA polymerase.I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.