The finding that aggregates of disease-unrelated proteins can induce cell death indicates that proteins may act as potential toxins. Cells experiencing toxic protein aggregates often display modifications of the redox status and ion balance, eventually leading to apoptosis; however, in some cases, tissue and cultured cell types die with features of necrosis. To elucidate the pathways leading to such different outcomes, we studied the biochemical features of death in HEND and NIH/3T3 cells exposed to prefibrillar aggregates of a disease-unrelated protein. The two types of cells died by apoptosis and necrosis, respectively. The pattern of caspase and proapoptotic factor activation was investigated together with the extent of mitochondria impairment and the energy load in either cell line. Our data depict a scenario where the events related to the extrinsic pathway of apoptosis are the same in the two cell lines, the difference in the final outcome being related to the extent of mitochondria derangement, possibly due to the different ability of the cells to counteract ion homeostasis impairment.

Patterns of cell death triggered in two different cell lines by HypF-N prefibrillar aggregates / M. BUCCIANTINI; S. RIGACCI; A. BERTI; L. PIERI; C. CECCHI; D. NOSI ; L. FORMIGLI; F. CHITI ; M. STEFANI. - In: THE FASEB JOURNAL. - ISSN 0892-6638. - STAMPA. - 19(3):(2005), pp. 437-439. [10.1096/fj.04-3086fje]

Patterns of cell death triggered in two different cell lines by HypF-N prefibrillar aggregates.

BUCCIANTINI, MONICA;RIGACCI, STEFANIA;BERTI, ANDREA;CECCHI, CRISTINA;NOSI, DANIELE;FORMIGLI, LUCIA;CHITI, FABRIZIO;STEFANI, MASSIMO
2005

Abstract

The finding that aggregates of disease-unrelated proteins can induce cell death indicates that proteins may act as potential toxins. Cells experiencing toxic protein aggregates often display modifications of the redox status and ion balance, eventually leading to apoptosis; however, in some cases, tissue and cultured cell types die with features of necrosis. To elucidate the pathways leading to such different outcomes, we studied the biochemical features of death in HEND and NIH/3T3 cells exposed to prefibrillar aggregates of a disease-unrelated protein. The two types of cells died by apoptosis and necrosis, respectively. The pattern of caspase and proapoptotic factor activation was investigated together with the extent of mitochondria impairment and the energy load in either cell line. Our data depict a scenario where the events related to the extrinsic pathway of apoptosis are the same in the two cell lines, the difference in the final outcome being related to the extent of mitochondria derangement, possibly due to the different ability of the cells to counteract ion homeostasis impairment.
2005
19(3)
437
439
M. BUCCIANTINI; S. RIGACCI; A. BERTI; L. PIERI; C. CECCHI; D. NOSI ; L. FORMIGLI; F. CHITI ; M. STEFANI
File in questo prodotto:
File Dimensione Formato  
Patterns of cell death.pdf

accesso aperto

Tipologia: Versione finale referata (Postprint, Accepted manuscript)
Licenza: Open Access
Dimensione 2.82 MB
Formato Adobe PDF
2.82 MB Adobe PDF

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/310204
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 45
  • ???jsp.display-item.citation.isi??? 39
social impact