This article deals with the solution structure determination of paramagnetic metalloproteins by NMR spectroscopy. These proteins were believed not to be suitable for NMR investigations for structure determination until a decade ago, but eventually novel experiments and software protocols were developed, with the aim of making the approach suitable for the goal and as userfriendly and safe as possible. In the article, we also give hints for the optimization of experiments with respect to each particular metal ion, with the aim of also providing a handy tool for nonspecialists. Finally, a section is dedicated to the significant progress made on 13C direct detection, which reduces the negative effects of paramagnetism and may constitute a new chapter in the whole field of NMR spectroscopy.

NMR spectroscopy of paramagnetic metalloproteins / I.Bertini; C.Luchinat; G.Parigi; R.Pierattelli. - In: CHEMBIOCHEM. - ISSN 1439-4227. - STAMPA. - 6:(2005), pp. 1536-1549. [10.1002/cbic.200500124]

NMR spectroscopy of paramagnetic metalloproteins

BERTINI, IVANO;LUCHINAT, CLAUDIO;PARIGI, GIACOMO;PIERATTELLI, ROBERTA
2005

Abstract

This article deals with the solution structure determination of paramagnetic metalloproteins by NMR spectroscopy. These proteins were believed not to be suitable for NMR investigations for structure determination until a decade ago, but eventually novel experiments and software protocols were developed, with the aim of making the approach suitable for the goal and as userfriendly and safe as possible. In the article, we also give hints for the optimization of experiments with respect to each particular metal ion, with the aim of also providing a handy tool for nonspecialists. Finally, a section is dedicated to the significant progress made on 13C direct detection, which reduces the negative effects of paramagnetism and may constitute a new chapter in the whole field of NMR spectroscopy.
2005
6
1536
1549
I.Bertini; C.Luchinat; G.Parigi; R.Pierattelli
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/310458
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