trova@unifi(opens in a new window)|View at Publisher| Export | Download | Add to List | More... Journal of the American Chemical Society Volume 124, Issue 3, 23 January 2002, Pages 374-375 Lanthanide modulation of the orientation of macromolecules induced by purple membrane (Article) Bernadó, P.a, Barbieri, R.b, Padrós, E.c, Luchinat, C.b, Pons, M.a a Departament de Química Orgànica, Universitat de Barcelona, Martí i Franquès, 1-11, 08028-Barcelona, Spain b Magnetic Resonance Center, University of Florence, Via Luigi Sacconi, 6, 50019 Sesto Fiorentino, Italy c Unitat de Biofísica, Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Barcelona, Spain View additional affiliations View references (25) Abstract Addition of Tb3+ to purple membrane (PM) suspensions changes the orientation of the menbrane normal from parallel to perpendicular with respect to the magnetic field. Residual dipolar couplings measured in protein L in the presence of PM are scaled by a factor of -1/2. NMR line broadening and cross-correlation effects induced by the addition of PM are partially reversed by Tb3+ but not by Tm3+ that has no effect on the orientation of PM. This is interpreted as the result of anisotropically restricted motion of protein L in the transiently PM-bound form.
Lanthanide modulation of the orientation of macromolecules induced by purple membrane / R. BARBIERI; C. LUCHINAT; P. BERNADO; E. PADROS; M. PONS. - In: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. - ISSN 0002-7863. - STAMPA. - 124:(2002), pp. 374-375. [10.1021/ja0170192]
Lanthanide modulation of the orientation of macromolecules induced by purple membrane
LUCHINAT, CLAUDIO;
2002
Abstract
trova@unifi(opens in a new window)|View at Publisher| Export | Download | Add to List | More... Journal of the American Chemical Society Volume 124, Issue 3, 23 January 2002, Pages 374-375 Lanthanide modulation of the orientation of macromolecules induced by purple membrane (Article) Bernadó, P.a, Barbieri, R.b, Padrós, E.c, Luchinat, C.b, Pons, M.a a Departament de Química Orgànica, Universitat de Barcelona, Martí i Franquès, 1-11, 08028-Barcelona, Spain b Magnetic Resonance Center, University of Florence, Via Luigi Sacconi, 6, 50019 Sesto Fiorentino, Italy c Unitat de Biofísica, Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Barcelona, Spain View additional affiliations View references (25) Abstract Addition of Tb3+ to purple membrane (PM) suspensions changes the orientation of the menbrane normal from parallel to perpendicular with respect to the magnetic field. Residual dipolar couplings measured in protein L in the presence of PM are scaled by a factor of -1/2. NMR line broadening and cross-correlation effects induced by the addition of PM are partially reversed by Tb3+ but not by Tm3+ that has no effect on the orientation of PM. This is interpreted as the result of anisotropically restricted motion of protein L in the transiently PM-bound form.
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