The Helicobacter pylori neutrophil-activating protein (HP-NAP) is a virulence factor of H. pylori that stimulates in neutrophils high production of oxygen radicals and adhesion to endothelial cells. We report here that HP-NAP is a TLR2 agonist able to induce the expression of IL-12 and IL-23 by neutrophils and monocytes. Addition in culture of HP-NAP, as an immune modulator, to antigen-induced T cell lines resulted in a remarkable increase in the number of IFN-gamma-producing T cells and decrease of IL-4-secreting cells, thus shifting the cytokine profile of antigen-activated human T cells from Th2 to a Th1 cytotoxic phenotype. We also found that in vivo HP-NAP elicited an antigen-specific Th1-polarized T cell response in the gastric mucosa of H. pylori-infected patients. These data indicate HP-NAP as an important factor of H. pylori able to elicit cells of the innate immune system to produce IL-12 and IL-23, and they suggest it as a new tool for promoting Th1 immune responses.

The Neutrophil Activating Protein of Helicobacter pylori promotes T helper type 1 immune responses / A. AMEDEI; CAPPON A.; CODOLO G.; CABRELLE A.; POLENGHI A.; BENAGIANO M.; TASCA E.; AZZURRI A.; D'ELIOS M.M.; DEL PRETE G.; DE BERNARD M.. - In: THE JOURNAL OF CLINICAL INVESTIGATION. - ISSN 0021-9738. - STAMPA. - 116:(2006), pp. 1092-1101. [10.1172/JCI27177]

The Neutrophil Activating Protein of Helicobacter pylori promotes T helper type 1 immune responses.

AMEDEI, AMEDEO;BENAGIANO, MARISA;AZZURRI, ANNALISA;D'ELIOS, MARIO MILCO;DEL PRETE, GIANFRANCO;
2006

Abstract

The Helicobacter pylori neutrophil-activating protein (HP-NAP) is a virulence factor of H. pylori that stimulates in neutrophils high production of oxygen radicals and adhesion to endothelial cells. We report here that HP-NAP is a TLR2 agonist able to induce the expression of IL-12 and IL-23 by neutrophils and monocytes. Addition in culture of HP-NAP, as an immune modulator, to antigen-induced T cell lines resulted in a remarkable increase in the number of IFN-gamma-producing T cells and decrease of IL-4-secreting cells, thus shifting the cytokine profile of antigen-activated human T cells from Th2 to a Th1 cytotoxic phenotype. We also found that in vivo HP-NAP elicited an antigen-specific Th1-polarized T cell response in the gastric mucosa of H. pylori-infected patients. These data indicate HP-NAP as an important factor of H. pylori able to elicit cells of the innate immune system to produce IL-12 and IL-23, and they suggest it as a new tool for promoting Th1 immune responses.
2006
116
1092
1101
A. AMEDEI; CAPPON A.; CODOLO G.; CABRELLE A.; POLENGHI A.; BENAGIANO M.; TASCA E.; AZZURRI A.; D'ELIOS M.M.; DEL PRETE G.; DE BERNARD M.
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/311529
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