The modulation of acetylcholine (ACh) release by 5-HT3 receptor activation was studied using in vivo microdialysis. Spontaneous and K1-stimulated ACh release were measured in frontoparietal cortex and hippocampus of freely moving rats. Two consecutive exposures to high K1 produced ACh release of similar magnitude. In the cortex, serotonin (5-HT) failed to alter spontaneous ACh release, but caused a concentration-dependent decrease of K1-evoked ACh release. Phenylbiguanide (PBG) and m-chlorophenylbiguanide, two selective 5-HT3 agonists, mimicked the 5-HT responses, but 8-hydroxy-2-(di-npropylamino) tetralin, a selective 5-HT1A agonist, was without effect. However, PBG failed to modify K1-evoked ACh release from the hippocampus. Systemic and local administration of a highly selective 5-HT3 antagonist, tropisetron ((3-atropanyl) 1H-indole-carboxylic acid ester) blocked the effect of both 5-HT and PBG. The inhibition of ACh release by PBG was sensitive to tetrodotoxin. These observations provide direct evidence that, in rat cortex, 5-HT modulates in-vivo release of ACh through activation of 5-HT3 receptors.

Serotonergic modulation of acetylcholine release from cortex of freely moving rats / M.G. GIOVANNINI; I.CECCARELLI; B. MOLINARI; M. CECCHI; J. GOLDFARB; P. BLANDINA. - In: THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS. - ISSN 0022-3565. - STAMPA. - 285:(1998), pp. 1219-1225.

Serotonergic modulation of acetylcholine release from cortex of freely moving rats

GIOVANNINI, MARIA GRAZIA;BLANDINA, PATRIZIO
1998

Abstract

The modulation of acetylcholine (ACh) release by 5-HT3 receptor activation was studied using in vivo microdialysis. Spontaneous and K1-stimulated ACh release were measured in frontoparietal cortex and hippocampus of freely moving rats. Two consecutive exposures to high K1 produced ACh release of similar magnitude. In the cortex, serotonin (5-HT) failed to alter spontaneous ACh release, but caused a concentration-dependent decrease of K1-evoked ACh release. Phenylbiguanide (PBG) and m-chlorophenylbiguanide, two selective 5-HT3 agonists, mimicked the 5-HT responses, but 8-hydroxy-2-(di-npropylamino) tetralin, a selective 5-HT1A agonist, was without effect. However, PBG failed to modify K1-evoked ACh release from the hippocampus. Systemic and local administration of a highly selective 5-HT3 antagonist, tropisetron ((3-atropanyl) 1H-indole-carboxylic acid ester) blocked the effect of both 5-HT and PBG. The inhibition of ACh release by PBG was sensitive to tetrodotoxin. These observations provide direct evidence that, in rat cortex, 5-HT modulates in-vivo release of ACh through activation of 5-HT3 receptors.
1998
285
1219
1225
M.G. GIOVANNINI; I.CECCARELLI; B. MOLINARI; M. CECCHI; J. GOLDFARB; P. BLANDINA
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/312592
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