Biochem Biophys Res Commun. 1990 Dec 14;173(2):528-33. Transformation by ras oncogene induces nuclear shift of protein kinase C. Chiarugi V, Magnelli L, Pasquali F, Vannucchi S, Bruni P, Quattrone A, Basi G, Capaccioli S, Ruggiero M. Source Laboratory of Molecular Biology, University of Firenze, Italy. Abstract We measured protein kinase C (PKC) activity in normal and ras-transformed Balb/3T3 fibroblasts; cytosolic and nuclear-associated PKC activity was determined either as phorbol ester binding, PKC-dependent phosphorylation of histone III-S, or phosphorylation of endogenous nuclear proteins. Results demonstrate that ras-transformed fibroblasts show down-regulation of cytosolic PKC accompanied by increase of nuclear-associated PKC. These results provide evidence linking transformation to PKC nuclear shift with consequent phosphorylation of nuclear proteins.

Transformation by ras oncogene induces nuclear shift of protein kinase C / V. CHIARUGI; L. MAGNELLI; F. PASQUALI; S. VANNUCCHI; P.BRUNI; A.QUATTRONE;G.BASI; S.CAPACCIOLI; M.RUGGIERO. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 173:(1990), pp. 528-533. [10.1016/S0006-291X(05)80066-X]

Transformation by ras oncogene induces nuclear shift of protein kinase C

CHIARUGI, VINCENZO;MAGNELLI, LUCIA;VANNUCCHI, SIMONETTA;BRUNI, PAOLA;QUATTRONE, ALESSANDRO;BASI, GABRIELE;CAPACCIOLI, SERGIO;RUGGIERO, MARCO
1990

Abstract

Biochem Biophys Res Commun. 1990 Dec 14;173(2):528-33. Transformation by ras oncogene induces nuclear shift of protein kinase C. Chiarugi V, Magnelli L, Pasquali F, Vannucchi S, Bruni P, Quattrone A, Basi G, Capaccioli S, Ruggiero M. Source Laboratory of Molecular Biology, University of Firenze, Italy. Abstract We measured protein kinase C (PKC) activity in normal and ras-transformed Balb/3T3 fibroblasts; cytosolic and nuclear-associated PKC activity was determined either as phorbol ester binding, PKC-dependent phosphorylation of histone III-S, or phosphorylation of endogenous nuclear proteins. Results demonstrate that ras-transformed fibroblasts show down-regulation of cytosolic PKC accompanied by increase of nuclear-associated PKC. These results provide evidence linking transformation to PKC nuclear shift with consequent phosphorylation of nuclear proteins.
1990
173
528
533
V. CHIARUGI; L. MAGNELLI; F. PASQUALI; S. VANNUCCHI; P.BRUNI; A.QUATTRONE;G.BASI; S.CAPACCIOLI; M.RUGGIERO
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/312740
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