Restriction endonuclease analysis was used as a new method to obtain genomic DNA fingerprints in yeast. Fifteen yeast strains belonging to the genera Saccharomyces and Zyggosaccharomyces were examined. Restriction fragments obtained with Apal or Kspl endonucleases were separated by SDS-PAGE and silver-stained. Analysis of the fingerprints showed that restriction enzyme digestion of genomic DNA can be successfully applied to yeast, enabling the differentiation between strains belonging to different or to the same species or genera.
DNA fingerprinting of yeast strains by restriction enzyme analysis / C. Barberio; R. Fani; A. Raso; A. Carli; M. Polsinelli. - In: RESEARCH IN MICROBIOLOGY. - ISSN 0923-2508. - STAMPA. - 145:(1994), pp. 659-666.
DNA fingerprinting of yeast strains by restriction enzyme analysis.
BARBERIO, CLAUDIA;FANI, RENATO;POLSINELLI, MARIO
1994
Abstract
Restriction endonuclease analysis was used as a new method to obtain genomic DNA fingerprints in yeast. Fifteen yeast strains belonging to the genera Saccharomyces and Zyggosaccharomyces were examined. Restriction fragments obtained with Apal or Kspl endonucleases were separated by SDS-PAGE and silver-stained. Analysis of the fingerprints showed that restriction enzyme digestion of genomic DNA can be successfully applied to yeast, enabling the differentiation between strains belonging to different or to the same species or genera.
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