The relaxation rate of water proton nuclei can be used to screen ligands (L; see picture) directed to the metal binding site in paramagnetic metalloproteins by NMR spectroscopy. The very high concentration of water protons provides an enormous sensitivity and permits the use of unprecedentedly low amounts of protein.
Water-based ligand screening for paramagnetic metalloproteins / I.Bertini; M.Fragai; C.Luchinat; E.Talluri. - In: ANGEWANDTE CHEMIE. INTERNATIONAL EDITION. - ISSN 1433-7851. - STAMPA. - 47:(2008), pp. 4533-4537. [10.1002/anie.200800327]
Water-based ligand screening for paramagnetic metalloproteins
BERTINI, IVANO;FRAGAI, MARCO;LUCHINAT, CLAUDIO;TALLURI, ELEONORA
2008
Abstract
The relaxation rate of water proton nuclei can be used to screen ligands (L; see picture) directed to the metal binding site in paramagnetic metalloproteins by NMR spectroscopy. The very high concentration of water protons provides an enormous sensitivity and permits the use of unprecedentedly low amounts of protein.
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