(13)C-(13)C NOESY experiments were performed under long mixing time conditions on reduced human superoxide dismutase (32 kDa, (15)N, (13C) and 70% (2)H labeled). (13)C-(13)C couplings were successfully eliminated through post-processing of in-phase-anti-phase (IPAP) data. It appears that at mixing time tau(m) of 3.0 s the spin diffusion mechanism allows the detection of 96% of the two-bond correlations involving C' and C(beta). The interpretation was confirmed by simulations. This approach broadens the range of applicability of (13)C-(13)C NOESY spectroscopy.

(13)C-(13)C NOESY: a constructive use of (13)C-(13)C spin-diffusion / I.Bertini; I.C.Felli; R.Kuemmerle; C.Luchinat; R.Pierattelli. - In: JOURNAL OF BIOMOLECULAR NMR. - ISSN 0925-2738. - STAMPA. - 30:(2004), pp. 245-251. [10.1007/s10858-005-1679-2]

(13)C-(13)C NOESY: a constructive use of (13)C-(13)C spin-diffusion

BERTINI, IVANO;FELLI, ISABELLA CATERINA;LUCHINAT, CLAUDIO;PIERATTELLI, ROBERTA
2004

Abstract

(13)C-(13)C NOESY experiments were performed under long mixing time conditions on reduced human superoxide dismutase (32 kDa, (15)N, (13C) and 70% (2)H labeled). (13)C-(13)C couplings were successfully eliminated through post-processing of in-phase-anti-phase (IPAP) data. It appears that at mixing time tau(m) of 3.0 s the spin diffusion mechanism allows the detection of 96% of the two-bond correlations involving C' and C(beta). The interpretation was confirmed by simulations. This approach broadens the range of applicability of (13)C-(13)C NOESY spectroscopy.
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245
251
I.Bertini; I.C.Felli; R.Kuemmerle; C.Luchinat; R.Pierattelli
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2158/320274
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