Abstract Acylphosphatase (AcP) is a low-molecular-weight protein widely distributed in many vertebrate tissues with a yet unknown physiologic function. To study the in vivo behavior of AcP, HeLa cells were transiently transfected with a vector expressing the AcP/EGFP fusion protein. Analysis of the transfected cells showed a high level of cellular death in cells expressing the AcP/EGFP fusion protein with respect to control cells expressing EGFP alone. Flow cytometry and time lapse analysis of AcP/EGFP transfected cells evidenced a typical pattern of apoptosis. Surprisingly, cells transfected with a mutated form of AcP, with negligible in vitro acylphosphatase activity, undergo apoptosis as well as cells transfected with wild-type protein, suggesting that the physiologic role of AcP could be not related to this catalytic activity.

Acylphosphatase Is a Strong Apoptosis Inducer in HeLa Cell Line / E. Giannoni; P. Cirri; P. Paoli; T. Fiaschi; G. Camici; G. Manao; G. Raugei; G. Ramponi. - In: MOLECULAR CELL BIOLOGY RESEARCH COMMUNICATIONS. - ISSN 1522-4724. - STAMPA. - 3:(2000), pp. 264-270. [doi:10.1006/mcbr.2000.0228]

Acylphosphatase Is a Strong Apoptosis Inducer in HeLa Cell Line

GIANNONI, ELISA;CIRRI, PAOLO;PAOLI, PAOLO;FIASCHI, TANIA;CAMICI, GUIDO;MANAO, GIAMPAOLO;RAUGEI, GIOVANNI;RAMPONI, GIAMPIETRO
2000

Abstract

Abstract Acylphosphatase (AcP) is a low-molecular-weight protein widely distributed in many vertebrate tissues with a yet unknown physiologic function. To study the in vivo behavior of AcP, HeLa cells were transiently transfected with a vector expressing the AcP/EGFP fusion protein. Analysis of the transfected cells showed a high level of cellular death in cells expressing the AcP/EGFP fusion protein with respect to control cells expressing EGFP alone. Flow cytometry and time lapse analysis of AcP/EGFP transfected cells evidenced a typical pattern of apoptosis. Surprisingly, cells transfected with a mutated form of AcP, with negligible in vitro acylphosphatase activity, undergo apoptosis as well as cells transfected with wild-type protein, suggesting that the physiologic role of AcP could be not related to this catalytic activity.
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E. Giannoni; P. Cirri; P. Paoli; T. Fiaschi; G. Camici; G. Manao; G. Raugei; G. Ramponi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2158/322084
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